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Table 1 Mutagenesis primers used to examine the role of the PMV replicase motif in virus accumulation.

From: Panicovirus accumulation is governed by two membrane-associated proteins with a newly identified conserved motif that contributes to pathogenicity

Mutant Primer Sequence (5'-3')a
F313A REP/F1-A CCCCAGCGGC TTCGTTCTTTGC
F1313F2 F1-313-F2 GGAACCCCAGCA AACTCGTTCTTTGC
P317A P317A-989R CTGTGGGTTTTGC AACCCCAGCG
N323A N323A-1007R CAGCCAACTGGGC AGCCTCTGTG
L325A L325A-1014R CTCCAGACAGCCGC CTGGTTAGC
Y330A REP/Y-A CACACCCTGTAGAGGGC TCTCCAG
C335A 1044R-C/A CCTTTCTTATCAGC CACCCTGTAGAG
F357A REP/F-A GGAATACAGC TGGCAAGGC
D363A REP/D-A TGATCCTGGG CGTATGCGC
P399A MUTPMV-1236R GCCCCAACTAATGC ATTGGTCACTAG
W405A REP/W-A CCAAGCAGTCGC ATTGGCCCC
  1. a. The altered nucleotides on the PMV cDNA are underlined.