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Figure 2 | Virology Journal

Figure 2

From: Panicovirus accumulation is governed by two membrane-associated proteins with a newly identified conserved motif that contributes to pathogenicity

Figure 2

Transfected foxtail millet protoplasts were harvested 2 days post-inoculation. (A) PMV transcripts inoculated alone or with SPMV. Transcripts KB238 and QP94 were co-inoculated with SPMV transcripts. The RNA was extracted and separated on TBE-agarose gels, blotted, and probed for PMV or SPMV accumulation with a 32P-labelled cDNA to detect PMV genomic (g) and subgenomic (sg) RNAs or SPMV RNA, respectively. (B) RNA blot of total RNA isolated from protoplasts inoculated with RT-Stop, Amb-Tyr, or both (RT-Stop + Amb-Tyr). Detection of PMV was as described for Panel A. PMV was used as a positive control and M represents mock-inoculated protoplasts.

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Virology Journal

ISSN: 1743-422X