Transfected foxtail millet protoplasts were harvested 2 days post-inoculation. (A) PMV transcripts inoculated alone or with SPMV. Transcripts KB238 and QP94 were co-inoculated with SPMV transcripts. The RNA was extracted and separated on TBE-agarose gels, blotted, and probed for PMV or SPMV accumulation with a 32P-labelled cDNA to detect PMV genomic (g) and subgenomic (sg) RNAs or SPMV RNA, respectively. (B) RNA blot of total RNA isolated from protoplasts inoculated with RT-Stop, Amb-Tyr, or both (RT-Stop + Amb-Tyr). Detection of PMV was as described for Panel A. PMV was used as a positive control and M represents mock-inoculated protoplasts.