Skip to main content
Figure 6 | Virology Journal

Figure 6

From: Nectin-2-mediated entry of a syncytial strain of herpes simplex virus via pH-independent fusion with the plasma membrane of Chinese hamster ovary cells

Figure 6

Entry and FFWO activities of HSV-1 strain HFEMsyn. As in the legend to Figure 1, syncytium formation of HFEMsyn was determined on wild type CHO (A), CHO-nectin-1 (B) or CHO-nectin-2 cells (C). Images represent MOIs of 1000 (A, C) or 1 (B). Effect of lysosomotropic agents on HFEMsyn entry. Virus entry into CHO-nectin-1 or CHO-nectin-2 cells in the presence of ammonium chloride (D) or monensin (E) was assayed as in the legend to Figure 2. Cells were infected with HFEMsyn at equivalent multiplicities based on the plating efficiency of HFEMsyn on the respective cell types. Based on Vero cell titer, this corresponds to MOIs of 1 and 450 for CHO nectin-1 and CHO-nectin-2 cells, respectively. Receptor-triggered FFWO of HFEMsyn. As in the legend to Figure 5, HFEMsyn was added to CHO-nectin-1 (F) or CHO-nectin-2 cells (G).

Back to article page

Virology Journal

ISSN: 1743-422X