Kinetics of ANG path entry via distinct entry routes. HSV-1 ANG path was bound to CHO-nectin-1 or CHO-nectin-2 cells in 24 well dishes for 1 h at 4°C (100 PFU/well). Cells were washed with PBS and then shifted to 37°C. At the indicated times post-infection, extracellular virus was inactivated by treatment with sodium citrate buffer (pH 3.0). Cells were washed with PBS and incubated for 24 h at 37°C. Cells were fixed in methanol:acetone, and syncytia were quantified by immunoperoxidase staining. Data shown are the mean of quadruplicate samples +/- standard deviation.