Figure 6From: The role of cholesterol and sphingolipids in chemokine receptor function and HIV-1 envelope glycoprotein-mediated fusionChemokine-Triggered Ca2+ Mobilization. Intracellular Ca2+ mobilization was measured by stimulating Fura-2 loaded NIH3T3CD4CXCR4 (A) or NIH3T3CD4CCR5 (B) cells with chemokines SDF1-α (A) or MIP1-β (B) at different concentrations. The ratio of fluorescence at 340 and 380 nm was calculated using a FL Win Lab program (Perkin-Elmer). Left panel: untreated cells, middle panel: PPMP-treated cells; right panel: MβCD-treated cells.Back to article page