Figure 1

Cholesterol Levels in NIH3T3 cells after MβCD treatment. NIH 3T3 cells were treated with 10 mM MβCD for 30 minutes at 37°C and cholesterol in total cell lysate was determined as described previously [13]. For the put-back experiments the cholesterol-depleted cells were incubated with an MβCD-cholesterol mixture for 60 minutes at 37°C according to [13].