Location of siRNA targets to the 5'UTR of HCV genome. Predicted secondary structure of the 5' UTR sequences (18–357). The sequence shown is that of the genotype 1a 5'UTR, HCV-H , and the structure based on previous studies [41-43]. Stem-loop structures are labeled for reference. The chimeric clones were made by fusing the GFP-encoding sequence, including a poly (A) tail, after the CCU sequence of the 5'UTR by overlapping PCR. The locations of siRNA targets in the stem-loop regions are shown by arrows.