Table 3 Amino acid sequence of the VV protein L3L and identified peptides Peptides detected from the Method 1 (SDS-PAGE + LC-ESI-Q-TOF MS), Method 2 (SDS-PAGE + LC-ESI-QIT MS), Method 3 (HPLC + LC-ESI-QIT MS), Method 4 (LC-ESI-Q-TOF MS), and Method 5 (MALDI-TOF/TOF MS) are denoted with an asterisk (*). Peptides that are in bold print have been identified by at least one of the five methods.
From: Pox proteomics: mass spectrometry analysis and identification of Vaccinia virion proteins
Method | MNTRTDVTNDNIDKNPTKRGDRNIPGRNERFNDQNRFNNDRPRPKPRLQP |
|---|---|
1 | Â Â Â Â ************** |
2 | Â Â Â Â ************** |
3 | Â |
4 | Â Â Â Â ************** |
5 | Â |
| Â | NQPPKQDNKCREENGDFINIRLCAYEKEYCNDGYLSPAYYMLKQVDDEEM |
1 | Â |
2 | Â |
3 | Â |
4 | Â |
5 | Â |
| Â | SCWSELSSLVRSRK AVGFPLLK AAKRISHGSMLYFEQLKNSKVVKLTPQV |
1 | Â |
2 | Â |
3 | Â |
4 | Â Â Â Â Â Â Â Â Â Â Â Â Â Â ******** |
5 | Â |
| Â | KCLNDTVIFQTVVILYSMYKRGIYSNEFCFDLVSIPRTNIVFSVNQLMFN |
1 | Â |
2 | Â |
3 | Â |
4 | Â |
5 | Â |
| Â | ICTDILVVLSICGNRLYRTNLPQSCYLNFIHGHETIARR GYEHSNYFFEW |
1 | Â |
2 | Â |
3 | Â |
4 | Â |
5 | Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â *********** |
| Â | LIKNHISLLTKQTMDILK VKKKYATGAPVNRLLEPGTLVYVPKEDYYFIG |
1 | Â |
2 | Â Â Â *************** |
3 | Â |
4 | Â Â Â Â Â Â Â Â Â Â Â ******* |
5 | *** |
| Â | ISLTDVSISDNVR VLFSTDGIVLEIEDFNIKHLFMAGEMFVR SQSSTIIV |
1 | Â |
2 | Â |
3 | Â |
4 | Â Â Â Â Â Â Â Â Â Â Â Â Â ****************** |
5 | Â Â Â Â Â Â Â Â Â Â Â Â Â ***************************** |