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Table 1 Vaccinia virion proteins identified in this study. Membrane- and core-enriched fractions were both analyzed by five different methods: Method 1 (SDS-PAGE + LC-ESI-Q-TOF MS), Method 2 (SDS-PAGE + LC-ESI-QIT MS), Method 3 (HPLC + LC-ESI-QIT MS), Method 4 (LC-ESI-Q-TOF MS), and Method 5 (MALDI-TOF/TOF MS). Identified proteins are listed according to their corresponding ORF. The total number of non-redundant peptides and the percent of the protein identified are recorded.

From: Pox proteomics: mass spectrometry analysis and identification of Vaccinia virion proteins

ORF Function/location Ref. Methods # peptides % Coverage
A3L Major core protein [17] 1,2,3,4,5 39 71.6
A4L IMV/P4a associated protein [18] 1,2,3,4,5 16 49.1
A5R RNA pol. subunit [19] 1,4,5 3 29.9
A7L Early transcription factor [20] 1,2,4,5 7 12.7
A10L Major core protein [21] 1,2,3,4,5 62 64.3
A12L Viral structural protein [22] 1,3,4 4 14.6
A13L Membrane phosphoprotein [23] 1,2,3,4,5 6 91.4
A14L Membrane phosphoprotein [23] 1,2,4,5 4 61.1
A15L Core assoc. protein [24] 1,2,3 5 60.2
A16L Myristoylprotein; entry/fusion [25, 26] 1,2,5 4 12.2
A17L IMV membrane prtn [27, 28] 1,2,4,5 4 32.0
A24R RNA pol. subunit [29] 1,2,4,5 26 30.9
A27L IMV membrane prtn [30] 1,2,3,4,5 17 70.0
A29L RNA pol. subunit [31] 2,5 2 8.2
A30L Virion component [32] 2,3,4,5 3 58.4
A33R EEV glycoprotein [33] 1,4,5 2 21.6
A34R EEV glycoprotein [34] 1,2,4 2 23.2
A42R Profilin homolog [35] 1,2,3,4,5 6 51.1
A46R Interact with host IL-1 [36] 1 2 12.6
A56R EEV glycoprtn, hemagglutinin [37] 1,4,5 3 12.4
B5R EEV glycoprotein [38] 4 2 10.4
B22R Serpin (C16L) [39] 1,2,4,5 3 19.9
D1R Capping enzyme subunit [40] 1,2,4,5 15 22.7
D2L virion component [41] 1,2,4,5 9 63.0
D3R virion component [41] 1,2,4,5 8 50.6
D6R Early transcription factor [42] 2,5 7 11.9
D8L IMV membrane protein [43, 44] 1,2,3,4,5 26 89.1
D11L DNA-dependent ATPase [45] 1,2,5 9 17.3
D12L Capping enzyme subunit [46] 1,2,4,5 8 40.4
E1L PolyA polymerase [47, 48] 2,4,5 4 11.1
E3L dsRNA dep. protein kinase [49] 1,5 1 13.2
E4L RNA polymerase [50] 1,2,4,5 5 27.8
E6R unknown   1,2,4,5 20 43.1
E8R Virion component [51, 52] 1,2,4,5 11 57.1
E10R Oxidase [53] 2,3,4,5 2 17.9
E11L Viral core protein [54] 1,2,4,5 2 26.4
F8L Cytosolic protein [55] 3,4,5 4 60.0
F9L Mem. prtn.; similarity to L1R [53] 1,2,3,5 4 22.6
F10L Protein kinase [56, 57] 1,2,5 4 15.7
F13L EEV membrane protein [58] 1,2,4,5 9 32.0
F17R DNA binding phosphoprotein [59] 1,2,3,4,5 9 55.4
G1L metalloproteinase [60] 1,2,4,5 10 19.1
G3L Entry/fusion complex [61] 2,3,4,5 6 41.4
G4L glutaredoxin [62] 2,3,4,5 11 77.4
G7L Core cmpnnt, partners w/A30L [63] 1,2,3,4,5 20 59.8
H1L Protein phosphatase [64] 1,2,3,4,5 10 67.3
H3L Immunodominant protein [65] 1,2,3,4,5 31 79.0
H4L RNA pol. associated protein [66] 1,2,4,5 5 10.6
H5R Membrane phosphoprotein [67] 1,2,3,4,5 8 49.3
I1L encapsidated DNA-binding prtn [68] 1,2,3,4,5 6 20.5
I3L DNA binding phosphoprotein [69, 70] 2,5 3 18.6
I5L Virion component [71] 1,4 5 94.9
I7L Core protein proteinase [72] 2 9 18.4
I8R RNA/DNA-dependant NTPase [73] 4,5 4 8.7
J1R IMV membrane protein [74] 1,2,4,5 5 30.1
J3R Poly(A) polymerase, RNA methyltransferase [48, 75] 1,2,4,5 14 47.4
J4R RNA polymerase [76] 1,2,4 6 38.4
J6R RNA polymerase [76] 1,2,4,5 34 33.9
K4L Homolog to VP37, phoshoplipase D [58, 77] 3,4 4 8.5
L1R IMV membrane protein [78] 2,3,4,5 8 40.8
L3L unknown   1,2,4,5 7 22.9
L4R Major core protein [79] 1,2,3,4,5 25 77.7
O2L Glutaredoxin [80, 81] 1,2,3,4,5 7 70.4