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Table 1 Vaccinia virion proteins identified in this study. Membrane- and core-enriched fractions were both analyzed by five different methods: Method 1 (SDS-PAGE + LC-ESI-Q-TOF MS), Method 2 (SDS-PAGE + LC-ESI-QIT MS), Method 3 (HPLC + LC-ESI-QIT MS), Method 4 (LC-ESI-Q-TOF MS), and Method 5 (MALDI-TOF/TOF MS). Identified proteins are listed according to their corresponding ORF. The total number of non-redundant peptides and the percent of the protein identified are recorded.

From: Pox proteomics: mass spectrometry analysis and identification of Vaccinia virion proteins

ORF

Function/location

Ref.

Methods

# peptides

% Coverage

A3L

Major core protein

[17]

1,2,3,4,5

39

71.6

A4L

IMV/P4a associated protein

[18]

1,2,3,4,5

16

49.1

A5R

RNA pol. subunit

[19]

1,4,5

3

29.9

A7L

Early transcription factor

[20]

1,2,4,5

7

12.7

A10L

Major core protein

[21]

1,2,3,4,5

62

64.3

A12L

Viral structural protein

[22]

1,3,4

4

14.6

A13L

Membrane phosphoprotein

[23]

1,2,3,4,5

6

91.4

A14L

Membrane phosphoprotein

[23]

1,2,4,5

4

61.1

A15L

Core assoc. protein

[24]

1,2,3

5

60.2

A16L

Myristoylprotein; entry/fusion

[25, 26]

1,2,5

4

12.2

A17L

IMV membrane prtn

[27, 28]

1,2,4,5

4

32.0

A24R

RNA pol. subunit

[29]

1,2,4,5

26

30.9

A27L

IMV membrane prtn

[30]

1,2,3,4,5

17

70.0

A29L

RNA pol. subunit

[31]

2,5

2

8.2

A30L

Virion component

[32]

2,3,4,5

3

58.4

A33R

EEV glycoprotein

[33]

1,4,5

2

21.6

A34R

EEV glycoprotein

[34]

1,2,4

2

23.2

A42R

Profilin homolog

[35]

1,2,3,4,5

6

51.1

A46R

Interact with host IL-1

[36]

1

2

12.6

A56R

EEV glycoprtn, hemagglutinin

[37]

1,4,5

3

12.4

B5R

EEV glycoprotein

[38]

4

2

10.4

B22R

Serpin (C16L)

[39]

1,2,4,5

3

19.9

D1R

Capping enzyme subunit

[40]

1,2,4,5

15

22.7

D2L

virion component

[41]

1,2,4,5

9

63.0

D3R

virion component

[41]

1,2,4,5

8

50.6

D6R

Early transcription factor

[42]

2,5

7

11.9

D8L

IMV membrane protein

[43, 44]

1,2,3,4,5

26

89.1

D11L

DNA-dependent ATPase

[45]

1,2,5

9

17.3

D12L

Capping enzyme subunit

[46]

1,2,4,5

8

40.4

E1L

PolyA polymerase

[47, 48]

2,4,5

4

11.1

E3L

dsRNA dep. protein kinase

[49]

1,5

1

13.2

E4L

RNA polymerase

[50]

1,2,4,5

5

27.8

E6R

unknown

 

1,2,4,5

20

43.1

E8R

Virion component

[51, 52]

1,2,4,5

11

57.1

E10R

Oxidase

[53]

2,3,4,5

2

17.9

E11L

Viral core protein

[54]

1,2,4,5

2

26.4

F8L

Cytosolic protein

[55]

3,4,5

4

60.0

F9L

Mem. prtn.; similarity to L1R

[53]

1,2,3,5

4

22.6

F10L

Protein kinase

[56, 57]

1,2,5

4

15.7

F13L

EEV membrane protein

[58]

1,2,4,5

9

32.0

F17R

DNA binding phosphoprotein

[59]

1,2,3,4,5

9

55.4

G1L

metalloproteinase

[60]

1,2,4,5

10

19.1

G3L

Entry/fusion complex

[61]

2,3,4,5

6

41.4

G4L

glutaredoxin

[62]

2,3,4,5

11

77.4

G7L

Core cmpnnt, partners w/A30L

[63]

1,2,3,4,5

20

59.8

H1L

Protein phosphatase

[64]

1,2,3,4,5

10

67.3

H3L

Immunodominant protein

[65]

1,2,3,4,5

31

79.0

H4L

RNA pol. associated protein

[66]

1,2,4,5

5

10.6

H5R

Membrane phosphoprotein

[67]

1,2,3,4,5

8

49.3

I1L

encapsidated DNA-binding prtn

[68]

1,2,3,4,5

6

20.5

I3L

DNA binding phosphoprotein

[69, 70]

2,5

3

18.6

I5L

Virion component

[71]

1,4

5

94.9

I7L

Core protein proteinase

[72]

2

9

18.4

I8R

RNA/DNA-dependant NTPase

[73]

4,5

4

8.7

J1R

IMV membrane protein

[74]

1,2,4,5

5

30.1

J3R

Poly(A) polymerase, RNA methyltransferase

[48, 75]

1,2,4,5

14

47.4

J4R

RNA polymerase

[76]

1,2,4

6

38.4

J6R

RNA polymerase

[76]

1,2,4,5

34

33.9

K4L

Homolog to VP37, phoshoplipase D

[58, 77]

3,4

4

8.5

L1R

IMV membrane protein

[78]

2,3,4,5

8

40.8

L3L

unknown

 

1,2,4,5

7

22.9

L4R

Major core protein

[79]

1,2,3,4,5

25

77.7

O2L

Glutaredoxin

[80, 81]

1,2,3,4,5

7

70.4

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Virology Journal

ISSN: 1743-422X