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Table 1 Sequential 3' deletions of pS2CAT198 and pS2CAT96 ssRNAs

From: The 3' sequences required for incorporation of an engineered ssRNA into the Reovirus genome

cDNA Clone Length of 5' S2 sequence Length of 3' S2 sequence CAT Activity [pg/ml] ssRNA detected dsRNA detected Engineered RNA incorporated into infectious virus
pS2CAT198 198 284 64 + + +
pS2CAT96 96 284 64 + + +
pS2CAT3a 96 250 64 + + +
pS2CAT3b 96 200 32 + + +
pS2CAT3c 96 150 32 + + +
pS2CAT3d 96 100 64 + + +
pS2CAT3e 96 50 64 + - -
pS2CAT3f 96 31 32 + - -
pS2CAT3g 96 75 64 + - -
pS2CAT3h 96 80 64 + - -
pS2CAT3i 96 90 32 + - -
pS2CAT3j 96 95 32 + - -
pS2CAT3k 96 96 64 + - -
pS2CAT3l 96 97 32 + - -
pS2CAT3m 96 98 64 + + +
pS2CAT3n 96 99 64 + + +
pS2CAT198l 198 97 64 + - -
pS2CAT198k 198 96 64 + - -
pS2CAT198j 198 95 32 + - -
pS2CAT198i 198 90 64 + - -
pS2CAT198h 198 80 64 + - -
pS2CAT198g 198 75 32 + - -
pS2CAT198e 198 50 32 + - -
pS2CAT198f 198 31 32 + - -