The 3' sequences required for incorporation of an engineered ssRNA into the Reovirus genome

Table 1 Sequential 3' deletions of pS2CAT198 and pS2CAT96 ssRNAs

cDNA Clone	Length of 5' S2 sequence	Length of 3' S2 sequence	CAT Activity [pg/ml]	ssRNA detected	dsRNA detected	Engineered RNA incorporated into infectious virus
pS2CAT198	198	284	64	+	+	+
pS2CAT96	96	284	64	+	+	+
pS2CAT3a	96	250	64	+	+	+
pS2CAT3b	96	200	32	+	+	+
pS2CAT3c	96	150	32	+	+	+
pS2CAT3d	96	100	64	+	+	+
pS2CAT3e	96	50	64	+	-	-
pS2CAT3f	96	31	32	+	-	-
pS2CAT3g	96	75	64	+	-	-
pS2CAT3h	96	80	64	+	-	-
pS2CAT3i	96	90	32	+	-	-
pS2CAT3j	96	95	32	+	-	-
pS2CAT3k	96	96	64	+	-	-
pS2CAT3l	96	97	32	+	-	-
pS2CAT3m	96	98	64	+	+	+
pS2CAT3n	96	99	64	+	+	+
pS2CAT198l	198	97	64	+	-	-
pS2CAT198k	198	96	64	+	-	-
pS2CAT198j	198	95	32	+	-	-
pS2CAT198i	198	90	64	+	-	-
pS2CAT198h	198	80	64	+	-	-
pS2CAT198g	198	75	32	+	-	-
pS2CAT198e	198	50	32	+	-	-
pS2CAT198f	198	31	32	+	-	-

ISSN: 1743-422X