Figure 2
From: The 3' sequences required for incorporation of an engineered ssRNA into the Reovirus genome
Detection of virus-generation intermediates using the ssRNAs generated from the sequence-substitution cDNA templates outlined in Table 2, using the reovirus infectious RNA system. The ssRNA (in the top panel), the dsRNA (in the next panel down) and the CAT dsRNA (the third panel down) are shown utilizing Northerns analyzing RNA extracted from cells lipofected 12 hours earlier with 9 wildtype ssRNAs and the ssRNA obtained following transcription of the indicated cDNA template. The fourth and bottom panel is an autoradiogram following SDS-PAGE generated by in vivo labeling with 32P of the dsRNA genome segments of an isolated progeny virus containing the indicated mutated-S2 dsRNA. Progeny virus generated following lipofection was first triple-plaque purified.