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Figure 2 | Virology Journal

Figure 2

From: Importance of disulphide bonds for vaccinia virus L1R protein function

Figure 2

(A) Diagram of the location of the six cysteine residues in L1R. (B) Transient Expression of L1R cysteine-to-serine single mutants. Infections were performed by transfection of 2 μg of pL1R each containing a single Cysteine-to-Serine mutation in the coding sequence at time of infection with VV-TetO:L1R at an MOI of 0.1 under non-permissive conditions. Infections were harvested at 24 hpi and titered on BSC40 cells. (C) Transient Expression of L1R cysteine-to-serine double mutants. Infections were performed by transfection of 2 μg of pL1R containing double Cysteine-to-Serine mutations in the coding sequence at time of infection with VV-TetO:L1R at an MOI of 0.1 under non-permissive conditions. Infections were harvested at 24 hpi and titered on BSC40 cells.

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