Treatment | Effect§ |
|---|
None | F | F | F |
IM28 | F (0.60) | P (1.83) | I (6.43) |
Dexamethasone | F (0.48) | P (1.67) | I (5.20) |
Con A | F (0.09) | P (0.22) | I (0.79) |
Heparin | F (2.70) | P (7.00) | I (22.0) |
Suramin | F (1.57) | P (3.90) | I (15.0) |
Dextran Sulfate 10,000 | F (0.02) | P (0.06) | I (0.20) |
Dextran Sulfate 500,000 | F (0.37) | P (1.15) | I (3.52) |
- §F = 50–60% of the surface is covered by syncytia; P = partial inhibition of fusion: < 10% of the surface is covered by syncytia; I = inhibition of syncytia formation.
- TF228.1.16 cells were mixed with 293/CD4+ cells (1:1 cell ratio) and transferred to a 24-well plate (105 cells per well in 200 μl of culture medium). TF228.1.16 cells and 293/CD4+ cells were incubated in the presence or absence of the drug (the final concentration in μg/ml is indicated in parenthesis) for 18 h. Following co-culture, three random fields of cells were photographed (not shown) and percentage fusion was determined as previously described [10].
