Acute ethanol inhibits the replication of other HCV replicon lines. 9–13, and 5–15-replicon cell lines were treated with 0, 100, or 200 mM of ethanol, and HCV RNA (panel A) and protein (panel B) was quantitated by real time RT-PCR and western blot analysis as described above. B., quantitation of changes in HCV NS3 and NS5A protein expression. Scanned blots were analyzed with Image J. For each lane, pixel intensities of NS3 and NS5A bands were normalized to the total Stat1 pixel intensity, and the percent change relative to untreated cells was calculated.