Figure 5From: A general method for nested RT-PCR amplification and sequencing the complete HCV genotype 1 open reading frameResolving discordant sequencing traces. A section of six overlapping primary sequencing traces is shown. Traces 1–4 clearly indicate nt 1270 (shaded) is a G, whereas traces 5 and 6 are ambiguous at this position because both G and A were detected. The nucleotide was manually identified as a G due to the predominance of G's among the six traces.Back to article page