Efficiency of replication compartment formation. Cells containing or lacking quiescent KM110-R were superinfected with n212 or KOS as described in the legend to figure 5, then examined for viral replication compartments by visualizing the intranuclear distribution of ICP4 (figure 5). Cells exhibiting large ICP4 structures that filled an appreciable fraction of the nucleus were scored as positive while cells displaying diffuse or small punctate ICP4 structures were scored as negative. 100–650 cells were scored for each treatment group in each experiment. The data presented are the average of three independent experiments. Bars represent the standard deviation.