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Figure 8 | Virology Journal

Figure 8

From: Involvement of PKR and RNase L in translational control and induction of apoptosis after Hepatitis C polyprotein expression from a Vaccinia virus recombinant

Figure 8

Expression of HCV polyprotein from VV induces apoptosis in HeLa and HepG2 cells that is caspase-dependent. A: HeLa cells were infected at 5 PFU/cell with the recombinant VT7-HCV7.9 individually or in combination (2.5 PFU of each virus/cell) with the recombinant VV-Bcl2 (inducibly expressing the anti-apoptotic Bcl-2 polypeptide) or with a general caspase inhibitor, Z-VAD-FMK (Calbiochem) at 50 μM, in the presence (+) or absence (-) of IPTG. The apoptotic levels were determined at 24 h.p.i by ELISA. B: HepG2 cells were infected at 10 PFU/cell with the recombinant VT7-HCV7.9 individually or in combination (5 PFU of each virus/cell) with the recombinant VV-Bcl2 or with a general caspase inhibitor, Z-VAD-FMK (Calbiochem) at 50 μM, in the presence (+) or absence (-) of IPTG. The apoptotic levels were determined at 48 h.p.i by ELISA. VV-PKR infected cells in the presence (+) of IPTG were used as positive controls.

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Virology Journal

ISSN: 1743-422X