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Figure 5 | Virology Journal

Figure 5

From: Involvement of PKR and RNase L in translational control and induction of apoptosis after Hepatitis C polyprotein expression from a Vaccinia virus recombinant

Figure 5

Expression of the dominant negative eIF-2α-S51A mutant by VV-eIF2αNP rescues the translation inhibition induced by HCV polyprotein. BSC40 cells grown in 12-well plates were infected at a total of 9 PFU/cell with the viruses indicated in the presence or absence of IPTG (1.5 mM). At 18 h.p.i. the cells were metabolically labeled with [35S] Met-Cys Promix (50 μCi/mL) for 30 min. and analysed by SDS-PAGE (12%) and autoradiography.

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Virology Journal

ISSN: 1743-422X