Figure 3From: Involvement of PKR and RNase L in translational control and induction of apoptosis after Hepatitis C polyprotein expression from a Vaccinia virus recombinantExpression of HCV polyprotein inhibits the production of infectious VV. BSC40 cells were infected at 5 PFU/cell with the recombinant VT7-HCV7.9 in the presence or absence of IPTG. After the indicated times postinfection the cells were collected, centrifuged and resuspended in 300 μL of DMEM. After three freeze-thawing cycles, followed by sonication, the cell extracts were titrated in BSC40 cells. The experiment was performed two times in duplicate. Means and standard deviations are shown.Back to article page