Effects of IL-28A on CAP-dependent and HCV IRES-dependent translation. The GSB1 cells was transfected with control plasmid or plasmid pRL-HL, which has different luciferases directed by either CAP- or HCV IRES. After 48 hours of transfection, the cells were treated with varying doses of IL-28A. Cell extracts were made after 24 hours of incubation, followed by luciferase determination. The data represents the average of three independent experiments. The open column indicates CAP-mediated translation. The shadowed column indicates HCV IRES-mediated translation. The error bars indicate the variations of three independent assays.