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Figure 2 | Virology Journal

Figure 2

From: Hepatitis B virus X protein interacts with β5 subunit of heterotrimeric guanine nucleotide binding protein

Figure 2

Positive Yeast Colonies with Potential HBX Interacting Proteins. Comparison between control yeast colonies and positive colonies from the library screening that developed blue colour over a 5-day incubation period at 30°C. A1-5, B1-5, C1-5, D1-5, E1-5, F1, G6-10, H6-10, I6-10, J6-10, K6-10, L6: colonies that turn blue after α-galactosidase assay screening indicated positive interaction between bait and cDNA library. F2 and L7: control using bait plasmid cloned with HBX transformed into yeast strain Y187 to test if the bait alone led to transcriptional activation. α-galactosidase assay screening indicate negative interaction between bait and library. Colonies remained white. F3 and L8: control using negative control plasmid, pGBKT7-Lamin transformed into yeast strain Y187. α-galactosidase assay screening indicate negative interaction between bait and library. Colonies remained white. F4 and L9: Control using bait plasmid, pGBKT7 transformed into yeast strain Y187. α-galactosidase assay screening indicate negative interaction between bait and library. Colonies remained white.

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