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Figure 3 | Virology Journal

Figure 3

From: Constitutive expression of Atlantic salmon Mx1 protein in CHSE-214 cells confers resistance to Infectious Salmon Anaemia virus

Figure 3

Amplification, melting curve, and agarose gel electrophoresis of RT-PCR targeting a 220-bp product on ISAV segment 8 using total RNA from uninfected controls: normal CHSE-214 cells (M-O), CHSE-214 cells constitutively expressing ASMx1 (P-R) and the water only control (S-U). Samples were subjected to real-time RT-PCR with 50 cycle amplification. The arrows in O, R and U represent DNA markers in lane M; note the absence of the 220 bp-band on these agarose gels.

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