Figure 4
From: Human enterovirus 71 subgenotype B3 lacks coxsackievirus A16-like neurovirulence in mice infection
Detection of enterovirus genome sequences in infected newborn mice brain. At selected intervals post-inoculation (indicated by the number above each lane), mice were sacrificed (each mouse indicated by the alphabet above each lane) and RT-PCR was performed using an enterovirus specific primers. The presence of a 150 bp amplified DNA fragment indicates the presence of enterovirus genome, which was later confirmed by DNA sequencing.