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Table 1 S RBD mutants, expression levels and binding to ACE2.

From: The SARS Coronavirus S Glycoprotein Receptor Binding Domain: Fine Mapping and Functional Characterization

Mutant

Mutation

Expression

Binding

ASA

1

E327

98

83

123

2

K333

86

90

176

3*

K344

95

102

159

4

K390

104

1

44

5

D392

110

95

69

6

D393

30

100

10

7

K411

90

103

33

8

D414

120

130

113

9

D415

90

102

97

10*

R426

73

7

95

11

N427

100

111

121

12

D429

103

0

9

13

T431

131

64

59

14

K439

85

87

65

15

R441

10

15

3

16*

Y442

105

110

68

17*

R444

80

86

52

18

H445

124

103

113

19

K447

87

85

138

20

R449

96

101

178

21

F451

69

71

64

22

D454

50

4

25

23

I455

77

6

89

24

D463

87

81

70

25*

L472

95

99

172

26

N473

100

0

70

27

W476

80

76

126

28

F483

91

3

2

29

Q492

95

3

5

30

Y494

50

7

21

31

R495

97

19

7

32

E502

110

84

175

33

S17–276

90

0

 

34

S319–518

100

100

 
  1. The mutants that significantly decrease binding to ACE2 are shown in bold. The * denotes mutant residues that are naturally occurring in various SCV strains (see Fig. 6A). The binding and expression values for the individual mutants are expressed as a percentage of the value for the S319–518 (wt) that is assumed 100%. The values of accessible surface area (ASA, Å2) for mutant residues were calculated from the crystal structure of the S RBD-ACE2 complex (coordinates provided by S. Harrison) by using the Lee and Richards' algorithm [23] with a probe radius of 1.4 Å.