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Table 1 S RBD mutants, expression levels and binding to ACE2.

From: The SARS Coronavirus S Glycoprotein Receptor Binding Domain: Fine Mapping and Functional Characterization

Mutant Mutation Expression Binding ASA
1 E327 98 83 123
2 K333 86 90 176
3* K344 95 102 159
4 K390 104 1 44
5 D392 110 95 69
6 D393 30 100 10
7 K411 90 103 33
8 D414 120 130 113
9 D415 90 102 97
10* R426 73 7 95
11 N427 100 111 121
12 D429 103 0 9
13 T431 131 64 59
14 K439 85 87 65
15 R441 10 15 3
16* Y442 105 110 68
17* R444 80 86 52
18 H445 124 103 113
19 K447 87 85 138
20 R449 96 101 178
21 F451 69 71 64
22 D454 50 4 25
23 I455 77 6 89
24 D463 87 81 70
25* L472 95 99 172
26 N473 100 0 70
27 W476 80 76 126
28 F483 91 3 2
29 Q492 95 3 5
30 Y494 50 7 21
31 R495 97 19 7
32 E502 110 84 175
33 S17–276 90 0  
34 S319–518 100 100  
  1. The mutants that significantly decrease binding to ACE2 are shown in bold. The * denotes mutant residues that are naturally occurring in various SCV strains (see Fig. 6A). The binding and expression values for the individual mutants are expressed as a percentage of the value for the S319–518 (wt) that is assumed 100%. The values of accessible surface area (ASA, Å2) for mutant residues were calculated from the crystal structure of the S RBD-ACE2 complex (coordinates provided by S. Harrison) by using the Lee and Richards' algorithm [23] with a probe radius of 1.4 Å.