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Table 2 Sequence diversity (mean p-distance) among different genomic regions of DENV-3

From: Strategically examining the full-genome of dengue virus type 3 in clinical isolates reveals its mutation spectra

    Nucleotide Amino acid
Virus ID No. Region No of Clones No of change/total Mutation frequencya (10-3) A→G or U→Cb (%) Mean p-distancec(10-3) Range (10-3) No of change/total Mutation frequencya(10-3) Mean p-distancec(10-3) Range (10-3)
1F C/PrM 23 24/17733 1.4 66.7 2.67 0–6.7 13/5175 2.5 5 0–8.9
1H C/PrM 29 29/22359 1.3 69 2.45 0–6.7 17/6525 2.6 5.12 0–22.2
2H C/PrM 21 16/16191 1.0 81.3 2.03 0–6.7 9/4725 1.9 3.81 0–13.3
2F C/PrM 22 18/16962 1.1 72.2 2.17 0–6.7 10/4950 2.0 4 0–13.3
3H C/PrM 13 11/10023 1.1 72.7 1.81 0–4 7/2925 2.4 4.7 0–17.8
3F C/PrM 24 24/18504 1.3 75 2.55 0–8 13/5400 2.4 4.8 0–17.8
Mean ± std C/PrM 22   1.2 ± 0.15 72.8 ± 5.1 2.28 ± 0.3    2.3 ± 0.3 4.6 ± 0.5  
1F E/NS1 26 40/32240 1.2 72.5 2.23 0–6.5 21/10192 2.1 4.3 0–10.2
1H E/NS1 13 47/16250 2.9 61.7 3.82 0–6.4 17/5096 3.3 6.6 0–7.6
2H E/NS1 25 33/16250 2.0 93.9 3.88 0–5.6 34/9800 3.5 6.4 0–12.6
2F E/NS1 13 15/22103 0.7 66.7 3.18 0–2.8 4/5096 0.8 7.6 0–9
3H E/NS1 23 44/20240 2.2 72.7 3.83 0–4.8 55/9016 6.1 6.2 0–12.6
3F E/NS1 20 39/24960 1.6 74.4 2.56 0–6.8 20/7840 2.6 4.8 0–21.7
Mean ± std E/NS1 20   1.8 ± 0.8 73.7 ± 11 3.7 ± 0.7    3.1 ± 1.8 6 ± 1.2  
1F NS3 19 5/17005 0.3 60 0.9 0–3.5 8/4978 1.6 3.3 0–11.4
1H NS3 27 16/24165 0.7 56.3 1.2 0–4.6 11/7074 1.6 3.1 0–11.4
2H NS3 26 9/23270 0.4 33.3 1.2 0–4.6 6/6812 0.9 3.2 0–11.5
2F NS3 25 16/22375 0.7 40 2.4 0–5.8 12/6550 1.8 5.7 0–15.3
3H NS3 23 25/20585 1.2 16 1.6 0–4.6 6/6026 1.0 2.0 0–11.4
3F NS3 18 8/16110 0.5 62.5 1.0 0–4.6 4/4716 0.8 1.6 0–11.4
Mean ± std   23   0.6 ± 0.3 44.7 ± 18 1.4 ± 0.6    1.3 ± 0.4 3 ± 1.4  
1F NS5 13 29/23335 1.2 69 1.7 0–4.5 17/7748 2.2 4.1 0–8.4
1H NS5 17 32/30515 1.0 71.9 2.4 0–5.6 31/10132 3.1 3.5 0–8.4
2H NS5 18 51/32310 1.6 64.7 3.0 0–8.9 33/10728 3.1 6.1 0–18.5
2F NS5 25 60/44875 1.3 65 2.5 0–5.6 47/14900 3.2 3.6 0–8.4
3H NS5 16 64/28720 2.2 56.3 4.3 0–7.3 46/9536 4.8 6.8 0–15.2
3F NS5 26 69/46670 1.5 52.2 3.7 0–6.7 52/15496 3.4 6.5 0–11.8
Mean ± std   19   1.5 ± 0.4 63.2 ± 7.5 3.1 ± 0.9    3.3 ± 0.8 5.1 ± 1.5  
  1. aMutation frequency is defined as the proportion of mutations relative to the consensus nucleotide or amino acid sequence for each patient and calculated by dividing the number of mutations relative to consensus by the total number of nucleotides or amino acid sequenced in each sample.
  2. b Percentage of A→G or U→C transitional mutations
  3. c p-distance is calculated by pairwise comparison of nucleotide or amino acid sequences between clones by the program MEGA d Indicated the virus derived from mosquito inoculation by C6/36-passaged one virus of patient ID#1H.