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Table 4 The Oligonucleotide primers and conditions used for RT-PCR of full-length genome of DENV-3

From: Strategically examining the full-genome of dengue virus type 3 in clinical isolates reveals its mutation spectra

PCR Primera Sequence (5' → 3') Genome Positionb Size(nt)c
P1A AGT TGT TAG TCT RCG TGG 1–18 1181
CP1181B TCC ARG CAC CTT CAG ATG 1181–1199  
DC530A AAC AWR TGC ACC CTC 540–555 1164
CDC1694B TGC ATK GCT CCT TCT TGR 1694–1712  
P1259A GGC AAG GGA AGC TTG GTG ACA TGC GC 1259–1285 1244
CDC2503B GGG AGT CTG CTT GGA ATT 2503–2521  
DC2171A GCC ATT CTR GGW GAC ACC GCY TGG GA 2171–2197 1246
CDC3417B TCT CTT CTT TGT CMT TCA 3417–3435  
D3-3142A CCA AAG AGT CTA GCT GGT CC 3142–3162 1535
D3-4677B CAT TGT GCG TCA ACA CTG CC 4677–4697  
d3NS2B1A AGC TGG CCA CTG AAT GAG G 4124–4143 1562
D35686B CAA AAG TCT TCC TAC TAA GTT G 5686–5708  
D3-5443A GCC GCA ATT TTC ATG ACA 5443–5461 2034
D3-7477B AAC AGC TAT CGT GGT GTT CC 7477–7497  
d37246A AAG AAT CCA ACG GTG GAT GG 7246–7266 1454
d38750B TCC CTT GTG CAT AAT CTG GG 8750–8770  
d38501A CAG GCT CAG CCT CCT CC 8501–8518 1654
d310316B GCT TCT TCC GTA CTG TGG C 10316–10335  
d39991A CTT ACT GTC TGG AAC AGG G 9991–10010 648
d310688B GTT GAT TCA ACA GCA CCA TTC 10688–10709  
  1. a Primer names with A in the end indicate a viral-sense orientation; names with B in the end indicate a complementary sense orientation
  2. b Genome positions are given according to the published sequence of strain H87 of dengue virus serotype 3
  3. c nt indicated nucleotide