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Figure 4 | Virology Journal

Figure 4

From: Baculovirus-mediated promoter assay and transcriptional analysis of white spot syndrome virus orf427 gene

Figure 4

Time course RT-PCR analysis of orf427 during viral pathogenesis. A) Gene specific primer sets used in the RT-PCR analysis as previously reported [6,7]. B) Agarose gel electrophoresis of RT-PCR products. Total RNA was sampled at the indicated time points post infection and RT-PCR was performed using primer sets specific for ie1, dnapol, vp28, orf427, and β-actin gene, individually. M: kb DNA ladder from Stratagene.

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