Time course RT-PCR analysis of orf427 during viral pathogenesis. A) Gene specific primer sets used in the RT-PCR analysis as previously reported [6,7]. B) Agarose gel electrophoresis of RT-PCR products. Total RNA was sampled at the indicated time points post infection and RT-PCR was performed using primer sets specific for ie1, dnapol, vp28, orf427, and β-actin gene, individually. M: kb DNA ladder from Stratagene.