Detection of ORF427 in infected shrimp through Western blot analysis. Western blotting analysis for detection of the endogenic ORF427 in infected shrimp cells. Polyclonal antibody toward Orf427 was raised using the bacterially expressed partial Orf427 as antigen from Guinea pigs. 1. Protein marker; 2, total shrimp cellular extracts sampled from normal shrimp; 3, total shrimp cellular extracts sampled from WSSV-infected shrimp.