Figure 3From: Development of an in vitro cleavage assay system to examine vaccinia virus I7L cysteine proteinase activityProcessing kinetics of P25K. Samples were incubated at either 0°C (A), 25°C (B), 30°C (C), or 37°C (D) for up to 20 hrs, harvested at the indicated times and the reaction stopped by the addition of SDS sample buffer. Incubation temperature is indicated on the left and P25K precursor and 25K mature product are indicated on the right.Back to article page