Dissociation curves of amplicons used to identify HSV-1, HSV-2 and dual positive samples. No curves were observed in HSV negative subjects. Tm was recorded and compared with probe binding and sequence results. Collectively nine different peaks with Tm in the range 86.6 – 88.0°C could be observed. Three Tm representative of HSV-1, HSV-2 and dual positive samples confirmed by sequencing are shown with Tm indicated.