Figure 8

Defects in DNA synthesis associated with pUL114. Triplicate wells of HEL cells were infected at an MOI of 0.01 with Towne (black circles) or the isogenic deletion virus, UL114 KO tag, (shaded squares). Total DNA was harvested at the indicated times, and the genome copy number was determined with a TaqMan assay using a standard curve of virion DNA. Copy number was normalized to the quantity of input genomes determined at 2 hpi with error bars representing the standard deviation of the triplicate samples. (A) The log of the accumulated viral DNA copy number is shown versus time post infection. The wt exponential rate of accumulation (r) was determined to be approximately 0.2 h^-1, whereas the copy number of the mutant expanded only at a rate of about 0.1 h^-1. (B) Data for the first 24 h replotted on a linear scale show the delayed onset of DNA synthesis during the first duplication of the viral genome.