Figure 7

Recruitment of pUL114 to the nucleus by ppUL44. Plasmids expressing ppUL44 or pUL114-EGFP fusion proteins were transfected into primary fibroblast cells and visualized by immunofluorescent staining. In the first row of images, ppUL44 stained with Texas Red exhibited strong nuclear localization as evidenced by the colocalization with DAPI in the merged image (violet). The pUL114-EGFP fusion protein and a similar protein containing a 25 aa amino terminal deletion (green) both localized to the cytoplasm and are shown merged with DAPI staining (blue). In the second row of images, the coexpression of ppUL44 (Texas red), pUL114-EGFP (green) and a merged image show that ppUL44 can recruit pUL114 to the nucleus. In third row of images ppUL44 (Texas red) and pUL114-EGFP containing a 25 aa amino terminal deletion (green) did not colocalize to the nucleus when co-expressed.