Figure 6

Coprecipitation of pUL114 and ppUL44. (A) Primary foreskin fibroblast cells were infected either with ICP4-tagged recombinant viruses or Towne at an MOI of approximately 1 PFU/cell. Cells were lysed at 48 hpi, and extracts were immunoprecipitated with a monoclonal antibody to ppUL44 and separated on an SDS-PAGE gel. Proteins were transferred to a membrane and a monoclonal antibody to the ICP4 epitope was used to detect coprecipitated poteins in the immunoblot. (B) EGFP.373 and C1-114.373 cells were infected with AD169 at an MOI of 2 PFU/cell and harvested at 24 hpi. Fusion proteins were precipitated with a monoclonal antibody to EGFP, separated on non-denaturing SDS PAGE gels, transferred to nitrocellulose, and immmunoblotting was performed with monoclonal antibody to ppUL44. Arrows designate the specific bands.