Figure 5

Localization of pUL114 in infected HEL cells. Cells were infected with a recombinant virus with an epitope tag in the carboxyl terminus of UL114. Monolayers were fixed and stained with an anti-ppUL44 monoclonal antibody (FITC) and an anti-ICP4 mouse monoclonal antibody (Texas Red). Cells were fixed at 48 hpi and images of FITC, Texas Red, and a merged image with DAPI are shown(A-C). Cells were fixed at 72 hpi and images of FITC, Texas Red, and a merged are shown (D-F). All images were captured digitally and prepared in Adobe Photoshop.