Detection of myc-3amut1 in transfected Vero E6 cells by FACS and Western blot analysis. (A) FACS analysis of live cells transfected with myc-3a, myc-3amut1 and myc-GST. Cells were initially probed with anti-myc monoclonal antibodies followed by the corresponding FITC-conjugated antibody. (B) Expression levels of the myc-tagged proteins in cells used for the FACS experiment were determined by Western blot analysis. Probing was done with anti-myc polyclonal antibody.