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Table 1 Inhibition of NE activity in U937 cells.a

From: Neutrophil elastase, an acid-independent serine protease, facilitates reovirus uncoating and infection in U937 promonocyte cells

 

% NE Activityb

% Cat Activityc

Time post-treatment

NE inhibitor

E64

Baf

NH4Cl

E64

NE inhibitor

3 h

7.5 ± 5.0

84.3 ± 5.8

87.0 ± 9.0

88.3 ± 9.4

ND

ND

2 d

8.8 ± 3.5

89.5 ± 9.1

88.8 ± 10.0

86.3 ± 3.6

-4.7 ± 1.6

98.8 ± 3.9

  1. aU937 cells were treated with the indicated inhibitors for 3 h or 2 d.
  2. bNE activity was assessed using the colorimetric substrate MeOSuc-Ala-Ala-Pro-Val-ρNA and percent activity relative to untreated cells was calculated.
  3. c Cathepsin L and B activity were assessed using the fluorogenic substrate Z-Phe-Arg-MCA and percent activity relative to untreated cells was calculated.