|
% NE Activityb
|
% Cat Activityc
|
---|
Time post-treatment
|
NE inhibitor
|
E64
|
Baf
|
NH4Cl
|
E64
|
NE inhibitor
|
---|
3 h
|
7.5 ± 5.0
|
84.3 ± 5.8
|
87.0 ± 9.0
|
88.3 ± 9.4
|
ND
|
ND
|
2 d
|
8.8 ± 3.5
|
89.5 ± 9.1
|
88.8 ± 10.0
|
86.3 ± 3.6
|
-4.7 ± 1.6
|
98.8 ± 3.9
|
- aU937 cells were treated with the indicated inhibitors for 3 h or 2 d.
- bNE activity was assessed using the colorimetric substrate MeOSuc-Ala-Ala-Pro-Val-ρNA and percent activity relative to untreated cells was calculated.
- c Cathepsin L and B activity were assessed using the fluorogenic substrate Z-Phe-Arg-MCA and percent activity relative to untreated cells was calculated.