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Table 1 Inhibition of NE activity in U937 cells.a

From: Neutrophil elastase, an acid-independent serine protease, facilitates reovirus uncoating and infection in U937 promonocyte cells

  % NE Activityb % Cat Activityc
Time post-treatment NE inhibitor E64 Baf NH4Cl E64 NE inhibitor
3 h 7.5 ± 5.0 84.3 ± 5.8 87.0 ± 9.0 88.3 ± 9.4 ND ND
2 d 8.8 ± 3.5 89.5 ± 9.1 88.8 ± 10.0 86.3 ± 3.6 -4.7 ± 1.6 98.8 ± 3.9
  1. aU937 cells were treated with the indicated inhibitors for 3 h or 2 d.
  2. bNE activity was assessed using the colorimetric substrate MeOSuc-Ala-Ala-Pro-Val-ρNA and percent activity relative to untreated cells was calculated.
  3. c Cathepsin L and B activity were assessed using the fluorogenic substrate Z-Phe-Arg-MCA and percent activity relative to untreated cells was calculated.
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Virology Journal

ISSN: 1743-422X