| % NE Activityb | % Cat Activityc |
---|
Time post-treatment | NE inhibitor | E64 | Baf | NH4Cl | E64 | NE inhibitor |
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3 h | 7.5 ± 5.0 | 84.3 ± 5.8 | 87.0 ± 9.0 | 88.3 ± 9.4 | ND | ND |
2 d | 8.8 ± 3.5 | 89.5 ± 9.1 | 88.8 ± 10.0 | 86.3 ± 3.6 | -4.7 ± 1.6 | 98.8 ± 3.9 |
- aU937 cells were treated with the indicated inhibitors for 3 h or 2 d.
- bNE activity was assessed using the colorimetric substrate MeOSuc-Ala-Ala-Pro-Val-ρNA and percent activity relative to untreated cells was calculated.
- c Cathepsin L and B activity were assessed using the fluorogenic substrate Z-Phe-Arg-MCA and percent activity relative to untreated cells was calculated.