Figure 2

Effects of agents that raise vesicular pH on reovirus replication in U937 and L929 cells. A. U937 cells were pre-treated without (-; black bars) or with 300 μM E64 (E64; grey bars) in the presence or absence of 25 nM Baf or 20 mM NH₄Cl. Following pre-treatment, cells were infected with reovirus strain Lang at an MOI of 3 and viral yield was measured at 3 d.p.i as described in the legend to Fig. 1B. B. L929 cells were pre-treated without (-) or with E64, 25 nM Baf or 20 mM NH₄Cl. Pre-treated cells were infected with reovirus strain Lang at an MOI of 3 and viral yield was measured at 3 d p.i. as described in the legend to Fig. 1B.