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Figure 5 | Virology Journal

Figure 5

From: Application of FTA technology for sampling, recovery and molecular characterization of viral pathogens and virus-derived transgenes from plant tissues

Figure 5

RT-PCR amplification of three RNA viral pathogens recovered from diseased N. benthamiana leaves pressed on FTA cards. Traditional isolation methods (lanes 1–3) were compared to RNA eluted from leaf pressed onto FTA cards (lanes 4–6) (a) a 900 bp fragment of tobacco mosaic virus (b) 1.5 & 2.1 kb fragments of tobacco etch virus (c) 1.5 & 2.1 kb fragments of potato virus Y. In all cases, although generating signals of lower strength compared to traditional RNA isolation methods, RNA eluted from FTA cards proved suitable for detection by RT-PCR analysis. M: marker -C: positive control, W: water control.

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