Skip to main content
Figure 3 | Virology Journal

Figure 3

From: Application of FTA technology for sampling, recovery and molecular characterization of viral pathogens and virus-derived transgenes from plant tissues

Figure 3

PCR amplification from serial dilutions of viral DNA elution from FTA cards. Serial dilutions of plasmid DNA carrying known amounts of the B genomic component of East African cassava Cameroon virus (EACMCV) were mixed with leaf sap extract from healthy cassava plants and spotted onto FTA cards. Viral DNA was eluted from FTA cards and used as template for PCR amplification of a 550 bp fragment using primers EAB555F and EAB555R guided experimental design (Table 1). 0.2 μg DNA was used as template in the positive control lane (C+).

Back to article page

Virology Journal

ISSN: 1743-422X