Figure 7
From: Intracellular localization of Crimean-Congo Hemorrhagic Fever (CCHF) virus glycoproteins
Intracellular co-localization analysis of GFP-CCHFV G fusion proteins. 293T cells were transfected with a GFP-CCHFV GN fusion protein construct encoding 123 amino acids from the GN cytoplasmic tail (GFP GNC). Twenty-four hours later cells were fixed with methanol/acetone. A subsequent indirect immunofluorescence using the Golgi compartment marker mannosidase II was performed and analyzed via UV microscopy. The merged picture clearly demonstrates the Golgi localization of the fusion protein.