Figure 6

GFP-CCHFV G fusion proteins to identify Golgi and ER localization signals. BHK-21 and 293T cells were transfected with individual GFP-CCHFV G fusion proteins. Twenty-four hours post transfection cells were analyzed via UV microscopy. A: Schematic presentation of different GFP-CCHFV G fusion proteins. Green labeled parts represent the GFP protein, red and blue labeled parts show the fragments of the CCHFV G cytoplasmic tails C-terminal fused to the GFP gene. White boxes symbolize the predicted hydrophobic transmembrane domains (TM). Numbers represent the amino acid position from the CCHFV GPC; B: BHK-21 cell analyses; C: 293T cell analyses.