Figure 5
From: Intracellular localization of Crimean-Congo Hemorrhagic Fever (CCHF) virus glycoproteins
Subcellular fractionation studies of expressed CCHFV glycoproteins. CCHFV-infected or CCHFV G expression plasmid-transfected BHK-21 cells were used for a subcellular fractionation study to determine if CCHFV G proteins are membrane associated. The results shown are summarized from two independent experiments. M: membrane fraction, S: soluble fraction, N: CCHF nucleoprotein, Man II: Mannosidase II. For quantification individual protein bands were analyzed and compared using the software Quantity One (BioRad).