Figure 4

Intracellular co-localization studies of CCHFV glycoproteins and cellular compartment markers. BHK-21 cells were either infected with CCHFV or transfected with individual CCHFV glycoprotein expression plasmids. Twenty-four hours post infection/transfection cells were fixed with methanol/acetone and subsequently an indirect immunofluorescence assay was performed. DAPI-stained (for nucleus localization), Golgi or ER compartment stained and CCHFV G expression pictures were taken individually and subsequently merged to study the intracellular CCHFV G localization. a: CCHFV-infected cells stained for CCHF G_N and G_C proteins; b and c: CCHFV GPC expression plasmid-transfected cells stained for G_N or G_C, respectively; d: Cells transfected with CCHFV G_C expression plasmid stained with G_C-specific antibodies and co-stained with ER compartment marker calreticulin; e: CCHFV G_Ns-transfected cells stained with HA tag-specific antibodies; f: CCHFV G_Nl-transfected cells stained with HA tag-specific antibodies; g: CCHFV G_C expression pattern after co-transfection of CCHFV G_Nl and G_C.