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Table 3 History of HCV positivity for CIMM-HCV isolates1

From: Transmission of human hepatitis C virus from patients in secondary cells for long term culture

 

Sample

Month

#081

112 A

112 B

112 AB

PCLB T4

1

-

    

2

-

    

3

+

    

4

+

-

-

  

5

+

+

+

  

6

ND

+

+

  

7

ND

+

+

-

 

8

ND

+

+

+

-

9

ND

+

+

+

+

10

ND

ND

ND

ND

-

11

ND

-

-

ND

-

12

ND

+

+

ND

+

13

ND

+

+

ND

+

14

ND

+

+

ND

+

15

ND

+

+

-

+

16

ND

+

+

+

+

17

ND

+

+

+

+

18

ND

+

+

+

+

19

ND

+

+

+

+

20

ND

+

+

+

+

21

ND

+

+

+

+

22

ND

+

+

+

+

23

ND

+

+

+

+

24

ND

+

+

+

+

25

-

ND

ND

ND

ND

26

+

ND

ND

ND

ND

27

+

ND

ND

ND

ND

28

+

ND

ND

ND

ND

29

+

ND

ND

ND

ND

30

+

ND

ND

-

-

31

+

ND

ND

+

+

  1. 1Each sample represents a monthly harvest of cell culture supernatants that were tested for the presence of human HCV positive-strand RNA and the cell free transmission to fresh target cells. Each individual sample was stored in liquid nitrogen at various time points throughout our testing. CIMM-HCV has been carried for over 12 months as a primary culture and over 31 months as a transmitted virus into other cell types including T-Cells (112A), B-Cells (112B), non-committed lymphoid cells (112AB) and 4th serial transmission into immortalized cord B-cells (PCLB T4). Primary cells are the first B-cells infected with HCV isolated from the macrophages.