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Table 3 History of HCV positivity for CIMM-HCV isolates1

From: Transmission of human hepatitis C virus from patients in secondary cells for long term culture

  Sample
Month #081 112 A 112 B 112 AB PCLB T4
1 -     
2 -     
3 +     
4 + - -   
5 + + +   
6 ND + +   
7 ND + + -  
8 ND + + + -
9 ND + + + +
10 ND ND ND ND -
11 ND - - ND -
12 ND + + ND +
13 ND + + ND +
14 ND + + ND +
15 ND + + - +
16 ND + + + +
17 ND + + + +
18 ND + + + +
19 ND + + + +
20 ND + + + +
21 ND + + + +
22 ND + + + +
23 ND + + + +
24 ND + + + +
25 - ND ND ND ND
26 + ND ND ND ND
27 + ND ND ND ND
28 + ND ND ND ND
29 + ND ND ND ND
30 + ND ND - -
31 + ND ND + +
  1. 1Each sample represents a monthly harvest of cell culture supernatants that were tested for the presence of human HCV positive-strand RNA and the cell free transmission to fresh target cells. Each individual sample was stored in liquid nitrogen at various time points throughout our testing. CIMM-HCV has been carried for over 12 months as a primary culture and over 31 months as a transmitted virus into other cell types including T-Cells (112A), B-Cells (112B), non-committed lymphoid cells (112AB) and 4th serial transmission into immortalized cord B-cells (PCLB T4). Primary cells are the first B-cells infected with HCV isolated from the macrophages.