Figure 6

Western blot analysis of HCV proteins under non-reducing conditions. Proteins were isolated from cell culture supernatants. (A) Analysis of large molecular weight proteins from the cell culture supernatants of CIMM-HCV in various cell lines. Lane 1 uninfected transformed B-cells, lane 2 transformed B-cells infected with HCV, lane 3 neuronal precursor cells derived from the metencephalon infected with HCV, lane 4 total protein from HCV positive sera and lane 5 commercially engineered HCV core antigen (ViroGen). Other smaller bands in the Core antigen may be due to breakdown products or other contaminating proteins. (B) Analysis of low molecular weight proteins of CIMM-HCV cultured in various cell lines. Lane 1 total protein from HCV positive sera, lane 2 transformed B-cells infected with HCV, lane 3 neuronal precursor cells derived from the metencephalon infected with HCV, and lane 4 uninfected transformed B-cells.