Figure 6From: Characterization of the protease domain of Rice tungro bacilliform virus responsible for the processing of the capsid protein from the polyproteinInduction of the putative protease domain. Expression of peptides in E.coli. Numbers on the left are estimated sizes in kDa of the molecular weight marker. (A) Coomassie blue-stained gel of induced peptides in E.coli. Lane 1: pTr-PR; Lane 2: pTr-mPR. (B) Western blot performed on induced peptides using antibodies raised against RTBV (Ab-RTBV). Lane 3: pTr-PR; Lane 4: pTr-mPR. (C) Western blot performed on induced peptides using antibodies raised against PR domain (Ab-PR). Lane 5: pTr-mPR. Peptide PR could not be induced from pTr-PR. pTr-mPR induced a specific peptide of about 14 kDa, corresponding to the protease domain (with mutation), and recognized by Ab-PR.Back to article page