Quantitative assessment of the effect of uracil-DNA glycosylase on amplicon DNA degradation and RNA amplification in reverse transcription-PCR

Table 4 Effect of incubation time on DNA degradation and RNA detection in the presence of UNG^a

^a The UNG concentration was 0.25 units per 25 μl reaction and incubation before RT-PCR was performed at 30°C for the indicated times.
^b The undiluted RNA sample and the 1:10⁷ dilution of the DNA sample contained 250,000 copies of RNA or DNA, respectively
^c Control reactions did not contain UNG and were not incubated prior to RT-PCR
^d P < 0.05 by paired t-test compared to control values
^e Indicates that amplification was not detected through 40 cycles in each of three replicate reactions

ISSN: 1743-422X