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Table 2 Effect of UNG concentration on DNA degradation and RNA detectiona

From: Quantitative assessment of the effect of uracil-DNA glycosylase on amplicon DNA degradation and RNA amplification in reverse transcription-PCR

   0.1 U UNG 0.25 U UNG 0.5 U UNG 1.0 U UNG
Analyte (dil.) Controlc CT incr. CT incr. CT incr. CT incr.
RNA (undil.)b 21.26 22.30 1.04 22.79 1.53 23.09 1.83 23.86 2.60
RNA (1:10) 26.25 26.86 0.61 27.57 1.32 27.95 1.70 27.31 1.06
RNA (1:100) 31.99 32.52 0.53 33.15 1.16 33.47 1.48 34.04 2.05
RNA (1:1000) 35.08 35.25 0.17 36.73 1.65 36.86 1.78 No Ct -
DNA (1:107)b 21.53 26.56 5.03 35.04 13.51 No Ct - No Ct -
DNA (1:108) 24.53 30.24 5.71 38.34 13.81 No Ct - No Ct -
DNA (1:109) 28.01 33.38 5.37 No Cte - No Ct - No Ct -
DNA (1:1010) 30.82 36.44 5.62 No Ct - No Ct - No Ct -
Mean CT increase for RNA 0.59d   1.42d   1.70d   1.90d
Mean CT increase for DNA 5.43   13.66   N/A   N/A
  1. a Incubation was performed before RT-PCR at 30°C for 30 min at the indicated enzyme concentrations
  2. b The undiluted RNA sample and the 1:107 dilution of the DNA sample contained 250,000 copies of RNA or DNA, respectively
  3. c Control reactions did not contain UNG and were not incubated prior to RT-PCR
  4. d P < 0.05 by paired t-test compared to control values
  5. e Indicates that amplification was not detected through 40 cycles in each of three replicate reactions
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