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Figure 2 | Virology Journal

Figure 2

From: Origin-independent plasmid replication occurs in vaccinia virus cytoplasmic factories and requires all five known poxvirus replication factors

Figure 2

Viral protein requirements for plasmid replication. (A) Conditional lethal ts mutants. BS-C-40 cells were transfected with p716 and 24 h later were mock infected or infected with 3 PFU per cell of wild type VAC (WR) or ts mutant Cts 24, Cts 25, ts 185, or Cts 16 at permissive (31°C) and non-permissive (39.5°C) temperatures for 24 h. DNA was then isolated, digested with Dpn I and quantified by real-time PCR. (B) D4R deletion mutant. RKD4R and RK-13 cells were transfected with p716 and 24 h later were infected with 3 PFU of vD4-ZG. At 24 after infection, DNA was isolated, digested with Dpn I, and the amount of DNA quantified by real-time PCR.

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Virology Journal

ISSN: 1743-422X