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Figure 18 | Virology Journal

Figure 18

From: CODEHOP-mediated PCR – A powerful technique for the identification and characterization of viral genomes

Figure 18

Output of the web-based CODEHOP software predicting complementary strand CODEHOP PCR primers for the conserved "DMGL" motif of herpesvirus TS genes. The TS_E block from the BlockMaker output in Figure 17 was provided as input to the CODEHOP software [3] and the PCR primers derived from the complementary strand are shown. The predicted consensus amino acid sequence is shown and the DMGL motif is underlined in bold. The complementary strand CODEHOP PCR primer selected for use in amplifying unknown TS genes is underlined in bold. The 3' degenerate core is shown in lowercase letters and the (len)gth and (degen)eracy are indicated. The 5' consensus clamp is shown in uppercase letters and the score, (len)gth and predicted melting (temp)erature are indicated.

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Virology Journal

ISSN: 1743-422X